Affichage des résultats 61 à 80 sur 1640 au total
Thèse d'Etienne Rajon - Jeudi 18 décembre 2008 - 14h00 - Amphi du Département de Génie Chimique IUT A
Adenosine-to-inosine (A-to-I) editing is hypothesized to facilitate adaptive evolution by expanding proteomic diversity through an epigenetic approach. However, it is challenging to provide evidences to support this hypothesis at the whole editome level. In this study, we systematically characterized 2,114 A-to-I RNA editing sites in female and male brains of D. melanogaster, and nearly half of these sites had events evolutionarily conserved across Drosophila species. We detected strong signatures of positive selection on the nonsynonymous editing sites in Drosophila brains, and the beneficial editing sites were significantly enriched in genes related to chemical and electrical neurotransmission in Drosophila. By adopting a method originally designed to detect linkage disequilibrium of DNA mutations, we examined the editomes of ten metazoan species and detected extensive linkage of editing in Drosophila and cephalopods. The prevalent linkages of editing in these two clades, many of which are conserved between closely related species and might be associated with the adaptive proteomic recoding, are maintained by natural selection at the cost of genome evolution. Nevertheless, in worms and humans, we only detected modest proportions of linked editing events, the majority of which were not conserved. Furthermore, the linkage of editing in coding regions of worms and humans might be overall deleterious, which drives the evolution of DNA sites to escape promiscuous editing. Altogether, our results suggest that the linkage landscape of A-to-I editing has evolved during metazoan evolution. If time permitting, I will also talk about other work related to adaptive evolution at the post-transcriptional level.
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Behavioral flexibility to optimize individual fitness occurs in many species and is regulated by endocrine mechanisms. If flexibility in social behavior exists in both sexes, this can lead to changes in the social organization of an entire population. I define this phenomenon as "social flexibility". Social flexibility can be advantageous in a changing environment. For example the African striped mouse (Rhabdomys pumilio) can either live solitarily or form extended family groups. Group-living striped mice can benefit from improved thermoregulation (huddling in nests) and possibly increased vigilance against nest predators. I explored the ultimate reasons of social flexibility by testing two main hypotheses, 1. ecological constraints (measured as population density) leading to philopatry and group-living, and 2. reproductive competition within groups leading to dispersal and solitary-living. Striped mice always lived in groups during the non-breeding season when reproductive competition was absent, indicating that the benefits of group-living prevailed during this period. During the breeding season when reproductive competition occurred, striped mice left the communal group and started solitary breeding if vacant territories were available. However, they remained group-living under high population density, consistent with the ecological constraints hypothesis. Fitness consequences of social flexibility have been studied in detail in male striped mice following alternative reproductive tactics. Depending on ecological conditions, solitary living males (roamers) can be making the best of a bad job with low reproductive success, or they can be equal in success to the group-living tactic, or they can even be superior with high reproductive success. Endocrine mechanisms (prolactin, testosterone and corticosterone) underlie social flexibility in the striped mouse. Thus, changes in social behavior (behavioral adaptation) seem to be regulated by changes in hormone levels (physiological adaptation), maximizing individual reproductive success (evolutionary adaptation) under changing environmental conditions.
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