Showing results 6061 to 6080 on 6936 in total
J.R. Lobry, A. Necşulea
Bénédicte Lafay, Paul M. Sharp
Manolo Gouy, Christian Gautier, F. Milleret
The local protein composition of chromatin controls important processes such as transcription, replication and DNA repair, yet the diversity of chromatin and its distribution along chromosomes is still poorly characterized.Using DamID in Drosophila Kc cells, we generated high-resolution genome-wide binding maps of 53 chromatin proteins from a wide range of functional categories. For most of those proteins, no binding data was previously available.By constructing a non supervised classifier, we find that there are five principal chromatin types defined by unique yet overlapping combinations of proteins.Two types correspond to Polycomb and HP1-bound regions, respectively. The novel 'BLACK' chromatin type covers half of the genome and induces strong transcriptional repression on inserted transgenes. Remarkably, this chromatin type is devoid of the classic 'heterochromatin' proteins Polycomb and HP1. Thus, our data reveal the existence of a prominent repressive chromatin type that has largely been overlooked.Active genes are associated with one of the other two remaining combinations of proteins. H3K36 methylation is associated with only one of them, yet it was previously thought to mark every transcribed gene. In addition, active genes involved in growth and cell proliferation, and those involved in signal transduction are located in a distinct chromatin types.The five chromatin types modulate the interactions of transcription factors with DNA. We observe that most transcription factors bind their cognate motif only if it sits in the favored chromatin context. Our data rule out a simple exclusion mechanism but support a model whereby synergistic interactions target transcription factors to their binding site.Finally, genomic regions in the 5 chromatin types follow different evolutionary processes. The vast majority of synteny breaks with Drosophila pseudoobscura occurs in only one of the transcriptionally active types. Besides, the speed of evolution of genes located in that chromatin type is higher than for other types.In summary, our integrative approach identifies five major chromatin types, which are defined by unique combinations of proteins and have distinct functional properties.
Bertrand P Beauvoit, Sophie Colombié, Jean-Pierre Mazat, Christine Nazaret, Sabine Pérès
Christian Bauer, Isabelle Pavlakovic, Catherine Mercier, Jean-Michel Maury, Catherine Koffel, Pascal Roy, Jean-Luc Fellahi
Pascale C Chevret, Gauthier Dobigny
Pascale C Chevret, Catherine Hänni
L. Say
Lilia Brinza, José Viñuelas, Ludovic Cottret, Federica Calevro, Yvan Rahbé, Gérard Febvay, Gabrielle Duport, Stefano Colella, Andréane Rabatel, Christian Gautier, Jean-Michel Fayard, Marie-France Sagot, Hubert Charles
J.-G. Wang, J.A. Staessen, S. Franklin, R. Fagard, François Gueyffier
J.-Y. Bobin, C. Zinzindohoue, S. Isaac, M. Saadat, Pascal Roy
Maryline Pellerin, Sonia Said, Jean-Michel Gaillard
Alice de Boyer Des Roches, Isabelle Veissier, Emmanuelle Gilot-Fremont, Jacques Capdeville, Luc Mounier
Sandrine Charles, Aude Ratier, Virgile Baudrot, Gauthier Multari, Aurélie Siberchicot, Dan Wu, Christelle Lopes
V. Miele, Y. Slaoui, A. Rebai
Damien M. de Vienne
Damien M. de Vienne
Nurulhusna Ab Hamid, Neal Alexander, Remco Suer, Nazni Wasi Ahmed, Rose Nani Mudin, Topek Omar, Rahmat Dapari, Shahrom Nor Azian Che Mat Din, Roslinda Abdul Rahman, Ropiah Jaraee, Frederic Baur, Frederic Schmitt, Nick Hamon, Jason Richardson, Carole Langlois-Jacques, Muriel Rabilloud, Mitra Saadatian-Elahi