The goal of my research is to bring an evolutionary perspective to the study of symbiosis, through the understanding of molecular interactions between partners.
Animals live in symbiosis with controlled bacterial communities in contrasting and changing environments. Symbionts can impact the phenotype of their host positively (e.g., by providing nutrients or protecting their host from pathogens) or negatively (by imposing a fitness cost). These partner interactions evolve in response to different selection pressures, making the associations dynamic along the continuum between parasitism and mutualism, and may also vary the degree of dependence between partners. I am particularly interested in the evolution of these symbiotic associations in response to different selection pressures, and in characterizing the molecular mechanisms underlying these changes.
Understanding the interactions between the bedbug and its microbiota, to control its populations by breaking the symbiotic association
Bedbugs have increased significantly over the last ten years, mainly due to the increase in human migration and resistance to insecticides. In the short term, the study of resistance determinants (project of Julien Varaldi / Jean-Philippe David) will allow to target the use of insecticides. In parallel, we are studying the interactions between the bedbug Cimex lectularius and its obligate bacterium Wolbachia in order to break the symbiotic association and control bedbug populations by alternative methods to pesticides. Indeed, Wolbachia wCle bacteria synthesize B vitamins that are necessary for the development and fertility of bedbugs. Using transcriptomics, metabolomics and microscopy approaches, we are studying in detail the interactions between the insect and its nutritional symbionts, in order to identify molecular mechanisms involved in the regulation of bacterial density and in the dependence of the bedbug on its symbiont.
Rapid Evolution of symbiotic interaction in response to stress
Funding JCJC ANR 2017-2021 / RESIST Project / PhD Alexis Bénard
Symbionts can promote or constrain rapid host adaptation to environmental changes. In response to global changes (temperature, precipitation; air, water and soil quality; biological invasions...) and to anthropization (intensive agriculture; urbanization...), it is therefore important to study the impact of these changes at the holobiont scale, i.e., the host and its microbial communities. In the RESIST project, we study more precisely: to what extent do stresses associated with the presence of pesticide (paraquat) and/or virus (DCV) impact flies of the genus Drosophila melanogaster, directly or indirectly via an impact on their Wolbachia symbionts? How does the symbiotic association evolve and more particularly if the presence of Wolbachia allows a rapid adaptation to these stresses? And what molecular and metabolic mechanisms are involved in the response to viral and/or oxidative stress?
Impact of commensal and endosymbiotic bacteria on viral infection in insects
The complex relationships between different microbial communities and how these communities can impact their host remain poorly studied. In the ComEndoVir project, we therefore characterized the impact of polymicrobial associations on Drosophila physiology. To this end, we created an artificial holobiont model, composed of two commensal bacteria of the digestive tract (Lactobacillus plantarum and Acetobacter pomorum), the intracellular bacterium Wolbachia and the pathogenic virus Drosophila C virus (DCV), and measured several life history traits related to the physiology of the host and the microbes. We showed that the multipartite interactions detected within the host impact host physiology, with multiple bacterial interactions notably allowing for improved tolerance to viral infection.
Post-doc - Establishment and maintenance of the squid/vibrio symbiosis
2010-2014. University of Madison-Wisconsin, USA. Supervision : Margaret McFall-Ngai
During my post-doc, I worked on the mutualistic association between the sepiola Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri, acquired anew each generation from the surrounding water. V. fischeri represents less than 0.1% of the bacterioplankton, but surprisingly, it is the only bacterium able to efficiently colonize the light organ of the squid. As the initiation of symbiosis can be easily reproduced in the laboratory, this association constitutes a particularly interesting model to study, in real time, the initial communication between the two partners of the symbiotic association. Using comparative transcriptomics and functional approaches, I characterized the first molecular dialogue between the two partners, just after their first contact.
Another interesting question is to understand how this symbiotic association is maintained, since we observe a daily phenomenon of expulsion then recolonization of bacteria in the light organ. This daily rhythm is associated with a coordinated change in gene expression in both the host and the bacteria, and it has been shown that the symbiotic population is maintained under hypoxic conditions. My second project was therefore to understand how the oxidative environment controls, and is controlled, by the symbiosis.
PhD - Evolution of dependancy in Wolbachia symbioses
2005-2009. University Lyon 1, Lyon, France. Supervision : Fabrice Vavre
During my thesis, I worked on the intracellular bacterium Wolbachia, which induces a wide range of phenotypic effects on its hosts, from facultative reproductive parasitism in arthropods to obligate mutualism in nematodes.
The hymenopteran Asobara tabida is one of the few species where Wolbachia is required for the ovogenesis of its arthropod host. It thus constitutes a particularly relevant study model to investigate the mechanisms underlying this recent evolutionary transition.
I first investigated the variability of Wolbachia dependence within the genus Asobara. Using various transcriptomic approaches, I then characterized the molecular mechanisms involved in the dependence between A. tabida and Wolbachia, and highlighted processes associated with oxidative stress, programmed cell death, and development. Finally, I examined the extent to which Wolbachia impacts the physiology of its host by studying iron metabolism in various symbiotic associations.
These studies revealed that dependence is not always associated with the contribution of new functions. Rather, it may reflect compensatory mechanisms in the host, in response to physiological perturbations induced by the presence of the symbiont. More generally, these results invite us to consider the effects and consequences of the presence of symbionts beyond the mechanisms that allow their persistence within populations.
Display of 1 to 30 publications on 33 in total
Wolbachia load variation in Drosophila is more likely caused by drift than by host genetic factors
Peer Community in Evolutionary Biology .
Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization
Communications Biology . 4 ( 1 ) : 1-15
Experimental evolution of virulence and associated traits in a Drosophila melanogaster - Wolbachia symbiosis
Peer Community in Evolutionary Biology .
Stress & Symbiosis: Heads or Tails?
Frontiers in Ecology and Evolution . : 1-9
Symbiotic organs shaped by distinct modes of genome evolution in cephalopods
Proceedings of the National Academy of Sciences of the United States of America .
Involvement of a host Cathepsin L in symbiont-induced cell death
MicrobiologyOpen . 7 ( 5 )
Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
mSystems . 3 ( 5 )
Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
Ecology and Evolution . 8 ( 20 ) : 10067 - 10074
Impact of Wolbachia on oxidative stress sensitivity in the parasitic wasp Asobara japonica
PLoS ONE . 5 : 10
Influence of oxidative homeostasis on bacterial density and cost of infection in Drosophila–Wolbachia symbioses
Journal of Evolutionary Biology . 29 : 1211-1222
SNP calling from RNA-seq data without a reference genome: identification, quantification, differential analysis and impact on the protein sequence
Nucleic Acids Research .
The chemistry of negotiation: Rhythmic, glycan-driven acidification in a symbiotic conversation
Proceedings of the National Academy of Sciences of the United States of America . 112 ( 2 ) : 566 - 571
The oxidative environment: a mediator of interspecies communication that drives symbiosis evolution
Proceedings of the Royal Society B: Biological Sciences . 281 ( 1785 ) : 20133112 - 20133112
A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide
eLife . 3
The dual nature of haemocyanin in the establishment and persistence of the squid - vibrio symbiosis
Proceedings of the Royal Society B: Biological Sciences . 281 ( 1785 ) : 20140504
Microbial impacts on insect evolutionary diversification: from patterns to mechanisms
Current Opinion in Insect Science . 4 : 29--34
Animals in a bacterial world, a new imperative for the life sciences.
Proceedings of the National Academy of Sciences of the United States of America . 110 ( 9 ) : 3229-36
Initial symbiont contact orchestrates host-organ-wide transcriptional changes that prime tissue colonization.
Cell Host and Microbe . 14 ( 2 ) : 183-94
The first engagement of partners in the Euprymna scolopes - Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells
Environmental Microbiology . : n/a - n/a
Modulation of Symbiont Lipid A Signaling by Host Alkaline Phosphatases in the Squid-Vibrio Symbiosis
mBio . 3 ( 3 )
Influence of Wolbachia on host gene expression in an obligatory symbiosis.
BMC Microbiology . 12 ( Suppl 1 ) : 1-16
Evolution and control of host-microbe symbiosis in arthropods: an RNAseq-based transcriptomic analysis
7. International Wolbachia conference . 978-2-911320-44-6
Vertical and horizontal transmission drive bacterial invasion
Molecular Ecology . 20 ( 17 ) : 3496 - 3498
Does a parthenogenesis-inducing Wolbachia induce vestigial cytoplasmic incompatibility?
The Science of Nature Naturwissenschaften . 98 ( 3 ) : 175-80
Do variable compensatory mechanisms explain the polymorphism of the dependence phenotype in the Asobara tabida-wolbachia association?
Evolution - International Journal of Organic Evolution . 64 ( 10 ) : 2969-79
Wolbachia interferes with ferritin expression and iron metabolism in insects.
PLoS Pathogens . 5 ( 10 ) : e1000630
A new case of Wolbachia dependence in the genus Asobara: evidence for parthenogenesis induction in Asobara japonica
Heredity . 103 ( 3 ) : 248-256
Is symbiosis evolution influenced by the pleiotropic role of programmed cell death in immunity an development ?
Insect Symbiosis . 3 : 57-76
The effects of age at mating on female life-history traits in a seed beetle
Behavioral Ecology . 18 : 551-555